Catalog Number - 68304-25P

Cogent RP C4 Butyl™ is a reversed-phase HPLC stationary phase designed for the separation of hydrophobic to moderately hydrophobic compounds. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, it provides excellent reproducibility, reliable peak shape, and balanced retention characteristics. As a USP L26 equivalent phase, it is commonly used as an alternative for compendial methods and for applications where lower hydrophobic retention than C8 or C18 phases is preferred.

68304-25P is an HPLC column packed with 3 µm RP C4 Butyl™ particles featuring a 100 Å pore size in a 4.6 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity for highly demanding separations, while the 3 µm particles deliver improved efficiency and sharper peaks compared to traditional 5 µm packings. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.

This column is particularly useful for hydrophobic and neutral compounds analyzed by reversed-phase HPLC when the highest possible resolving power is required. The C4 chemistry provides lower retention than C8 phases, helping reduce excessive retention while maintaining excellent separation performance. The extended 250 mm column length is especially beneficial for impurity profiling, complex sample characterization, and methods requiring maximum chromatographic resolution.

Key Benefits

• RP C4 Butyl™ stationary phase

• USP L26 equivalent chemistry

• Fully double end capped

• Traditional butyl (C4) bonded phase

• Ultra-high purity silica support

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Maximum peak capacity

• Exceptional chromatographic resolution

• Lower retention than C8 columns

Typical Applications

• USP L26 methods

• Reversed-phase HPLC

• Small molecule analysis

• Hydrophobic compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Quality control testing

• Impurity profiling

• Complex sample separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4 Butyl™

• USP Classification: L26

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Suitable for conventional HPLC systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 250 mm length provides maximum resolution and peak capacity within the RP C4 Butyl™ product family

• Lower retention than comparable C8 stationary phases

• Well suited for hydrophobic and neutral compounds

• Ideal for impurity profiling and highly complex sample separations

• Commonly used as an alternative for USP L26 methods

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